Effects of Foliar Application of Zinc Oxide Nanoparticles on Morphophysiological and Bochemical Responses of Catharanthus Roseus (L.) G. Don under Water Deficit Stress

Haq Nari, Haniyeh; Rezaei Nejad, Abdolhossein; Beiranvand, Farhad; Zomorrodi, Nahid

doi:10.22077/jdcr.2025.10186.1176

Document Type : Original Article

Authors

¹ Ms.C. Graduate in Ornamental Plants, Department of Horticultural Sciences, Faculty of Agriculture, Lorestan University, Lorestan, Iran

² Professor of Ornamental Plant Physiology, Department of Horticultural Sciences, Faculty of Agriculture, Lorestan University, Lorestan, Iran

³ Ph.D. in Horticultural Sciences – Physiology of Ornamental Plants. Department of Horticultural Sciences, Faculty of Agriculture, Lorestan University, Lorestan, Iran

https://doi.org/10.22077/jdcr.2025.10186.1176

Abstract

The present study aimed to investigate the effects of foliar application of zinc oxide nanoparticles (ZnO-NPs) on the morphological and physio-biochemical traits of the ornamental–medicinal plant periwinkle [Catharanthus roseus (L.)] under water deficit stress. The experiment was conducted as a factorial arrangement based on a completely randomized design) with four replications. The first factor was water deficit at three levels (80%, 50%, and 20% of available water content), while the second factor consisted of foliar application of ZnO-NPs at four concentrations (0, 50, 100, and 200 µM), applied as a pre-treatment at the four-leaf stage. The experiment continued until full flowering (two months), after which morpho-physiological parameters (plant height, stem length and diameter, leaf number, root volume and length, fresh and dry weights of stems, leaves, and roots, gas exchange parameters, relative water content (RWC), and electrolyte leakage (EL)) as well as biochemical traits (malondialdehyde (MDA) content, proline content, photosynthetic pigments concentration, and the activities of catalase, peroxidase, and ascorbate peroxidase) were measured. The results demonstrated that water deficit stress significantly reduced plant height, leaf area, RWC, photosynthetic pigments concentration, fresh and dry biomass, and gas exchange, while increasing EL, MDA content, proline accumulation, and antioxidant enzyme activities. In contrast, foliar application of ZnO-NPs enhanced antioxidant enzyme activity, RWC, photosynthetic pigments, and gas exchange, thereby improving plant tolerance to drought stress. Among the tested concentrations, 50 and 100 µM ZnO-NPs were the most effective in alleviating the adverse effects of water deficit and promoting plant growth under stress conditions.

Keywords

Main Subjects

Drought

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Effects of Foliar Application of Zinc Oxide Nanoparticles on Morphophysiological and Bochemical Responses of Catharanthus Roseus (L.) G. Don under Water Deficit Stress

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Volume 4, Issue 1 - Serial Number 13May 2026Pages 203-226

Volume 4, Issue 1 - Serial Number 13
May 2026
Pages 203-226